Year: 2017 | Month: December | Volume 7 | Issue 6
Scanning Electron Microscopic Studies on the Uterus of Buffalo during Follicular and Luteal Phases of Estrous Cycle
Abstract:
The present investigation was aimed to elucidate the surface ultrastructure of uterine horn, body and cervix of buffalo during follicular and luteal phase of estrous cycle. The present study was conducted on uterus of buffaloes during follicular and luteal phase of estrous cycle. The tissue samples fixed in Karnovsky’s fixative and processed for viewing under scanning electron microscope. The luminal surface of uterus was folded into broad ridges and folds. The luminal surface was lined with ciliated and non-ciliated cells. Ciliated cells had many kinocilia during the follicular phase. The non-ciliated cells dominated both during follicular and luteal phases of estrous cycle. The non-ciliated cells were of two types viz: secretory and non-secretory type. The surface of the cervix was highly folded with ridges and deep crypts. A scanning electron microscopic examination of the cervix revealed exposed ridges composed of cell apices that alternate with deep folds or crypts. The exposed ridges consisted of the external apical membranes of the columnar epithelial cells that form the cervical lining. Ciliated cell surrounded by several non-ciliated cells from the posterior region of the cervix. The cells surrounding the ciliated cell were covered with much shorter projections or microvilli that characterize secretory cells. Ciliated cells terminated with a group of cilia that emerged from the apical cell membrane of the epithelial cells and projected towards the lumen of the cervix.
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